Figure 4
In-vitro wounding assay in 3T3-Swiss fibroblasts (n = 3, ± SD). The addition treatment of L-NAME to LPS increased the percentages of cellular recover area while the treatment of L-NAME alone decreased it in wounded 3T3-Swiss fibroblasts. When stimulated with LPS, EPA treated fibroblasts had higher the percentage of cellular recover area than the AA treated cells. Cells were incubated for 48 hr with bovine serum albumin alone as control or bovine serum albumin-soap loaded fatty acids (25 μM). Then wound was created and the medium was replaced with fresh fatty acid enriched medium containing lipopolysaccharide (LPS, 10 μg/ml), NG-nitro-L-arginine methyl ester (L-NAME, 10-7 M) and indomethacin (10-8 M) for another 24 hr. Multiple photographs of the wound were obtained and the percentage of cellular recover areas were determined. Bars with different letters are significantly different (P < 0.05). EPA, eicosapentaenoic acid; AA, arachidonic acid; LPS, lipopolysaccharide; L-NAME, NG-nitro-L-arginine methyl ester; INDO, indomethacin.