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Table 2 Collagen formation in 3T3-Swiss fibroblasts (n = 3, ± SD). Cells were incubated for 48 hr with bovine serum albumin alone as control or bovine serum albumin-soap loaded fatty acids (25 μM). The wound was then created and the medium was then replaced with fresh fatty acid enriched medium containing 50 μM ascorbic acid and 5 μCi of 3H-proline with or without lipopolysaccharide (LPS, 10 μg/ml) and NG-nitro-L-arginine methyl ester (L-NAME, 100 nM). A duplicate set of plates was made without wounding. Cells were harvested at 24 hr post wounding and assayed for collagen, total protein and DNA. The amount of collagen is expressed as DPM from 3H-proline per μg of DNA. Mean values within rows having different superscripts are significantly different (P < 0.05) by 1-way ANOVA and Tukey test. EPA, eicosapentaenoic acid; AA, arachidonic acid; LPS, lipopolysaccharide; L-NAME, NG-nitro-L-arginine methyl ester.

From: Inducible nitric oxide synthase links NF-κB to PGE2 in polyunsaturated fatty acid altered fibroblast in-vitro wound healing

Treatments Collagen production (CP) (DPM/μg DNA amount) Collagen as a percentage of total protein (C-PTP) (%)
Control 2419.6 ± 318.1a 18.7 ± 1.6a
Wound 3044.8 ± 269.2b 21.8 ± 1.5b
LPS 3953.3 ± 57.3c 26.5 ± 0.8c
L-NAME 1225.6 ± 270.7d 11.4 ± 2.7d
LPS + Wound 2650.3 ± 146.9ab 19.0 ± 1.8ab
LPS + L-NAME 5393.7 ± 583.4e 37.5 ± 3.3e
LPS + L-NAME + EPA 6580.2 ± 418.3e 42.4 ± 5.6e
LPS + L-NAME + AA 3511.3 ± 561.4c 26.7 ± 4.0c