5'-DI mRNA analysis in liver in different groups: Ia-Se deficient control, IIa-Se adequate control, IIIa-Se excess control, Ib-Se deficient+HCD, IIb-Se adequate+HCD, IIIb-Se excess+HCD after 1 and 2 months. (a) mRNA expression by RT-PCR. (b) expression was quantified by densitometric analysis. β-actin was used as an internal control. Data is expressed as mean ± SD from 4 observations. *p<0.05, **p<0.01 represent comparison between control and HCD groups; Ap<0.05 comparison between 1 and 2 months; Dp<0.05, DDp<0.01 comparison between group Ia and IIa; ##p<0.01 comparison between group Ib and IIb; Lp<0.05, LLLp<0.001 comparison between group IIa and IIIa; Bp<0.05, BBBp<0.001 comparison between group IIb and IIIb.