Hepatic LDL-R mRNA analysis in different groups: Ia-Se deficient control, IIa-Se adequate control, IIIa-Se excess control, Ib-Se deficient+HCD, IIb-Se adequate+HCD, IIIb-Se excess+HCD after 1 and 2 months. (a) mRNA expression by RT-PCR. β-actin was also amplified (house keeping gene). (b) bands were quantified by densitometric analysis. Data is expressed as mean ± SD from 4 observations. *p<0.05, **p<0.01, ***p<0.001 represent comparison between control and HCD groups; Ap<0.05, AAp<0.01, AAAp<0.001 comparison between 1 and 2 months; DDDp<0.001 comparison between group Ia and IIa; ###p<0.001 comparison between group Ib and IIb; LLp<0.01, comparison between group IIa and IIIa; BBBp<0.001 comparison between group IIb and IIIb.