Effect of 13-MTD on the expression of several regulatory genes involved in the apoptosis induction in SKBR-3 cells. Cells pre-cultured for 24 hours were treated with the fatty acids for 4 h, and gene expression were measure by real time PCR. The relative change in gene expression was quantified essentially based on the 2 -ΔΔCT methods. Data are mean ± 95% confidence intervals of triplicates analyses. Data not sharing the same letter shows the statistically significant difference at p < 0.05 by Tukey-Kramer test.