Effect of 13-MTD on cellular calcium mobilization in SKBR-3 cells. SKBR-3 cells were seeded into black 96-well plate at 105 cells/well, and incubated for 1 day. Cells were loaded with 4 μM Fluo 3-AM for 30 min, and subsequently stimulated with 0.125, 0.25 and 0.5 mM fatty acids in 0.8% Tween 80 in PBS. The increases in fluorescence were measured 25 times with 0.15 s intervals. Data are mean ± SE of 38 replicate analyses. Data not sharing the same letter are significantly different at p < 0.05 by Tukey-Kramer test.