Influence of CR and re-feeding on food intake (A) and body weight (B). Values are expressed as means and 4–8 mice were analyzed in each group. The black arrow indicates the initiation of CR on day 8. The white arrow indicates the initiation of ad libitum re-feeding on day 30. The * indicates that the food intake average of the days 38 to 50 of re-feeding for the R-RF mice was significantly less than their food intake prior to initiation of CR, as determined by the paired t-test.