Lipid analysis. Sulfatide (A) and galactosylceramide (B) levels in whole brain extracts of ASA-deficient mice with one or two intact CGT genes and wild type controls. No significant difference between the ASA-deficient mice with different CGT backgrounds could be observed at all examined time points. Lipid concentrations are given in μmol per gram homogenized brain powder. Lipids were quantified by thin layer chromatography. The numbers of animals used for analysis (n) are given inside the columns. Error bars represent standard deviations of the mean values. **P ≤ 0.01 knock out against wild type by 1-way ANOVA; n.s.: not significant.