Figure 4From: Syndecan-1 mediates internalization of apoE-VLDL through a low density lipoprotein receptor-related protein (LRP)-independent, non-clathrin-mediated pathwayExpression of Syn-1 restores apoE-VLDL uptake in GM00701 cells. GM00701 (panels A and C) and GM00701/Syn-1-HA (panels B and D) cells were incubated with DiI-labeled apoE-VLDL (4 μg/ml) for 3 h at 37°C. Unassociated ligand was removed by rinsing, cells were fixed with paraformaldehyde and observed by fluorescence microscopy (panels C and D, 550 nm excitation-573 nm emission). Corresponding phase contrast images are shown in panels A and B. Magnification, 630X.Back to article page