Figure 5

Comparison of ¹²⁵I-apoE-VLDL binding to GM00701 and GM00701/Syn-1-HA. GM00701 or GM00701/Syn-HA cells were incubated with ¹²⁵I-apoE-VLDL (2 μg/ml) for 3 h at 4°C in the absence or presence of unlabeled apoE-VLDL (100 μg/ml). Unbound ligand was removed by rinsing, cells were solubilized with 0.1 M NaOH and subjected to scintillation counting. Values shown represent specific ¹²⁵I-apoE-VLDL binding and were obtained by subtracting amounts of ¹²⁵I-apoE-VLDL bound in the presence of unlabeled apoE-VLDL from total ¹²⁵I-apoE-VLDL binding. Error bars represent standard deviations of triplicate points.