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Figure 3 | Lipids in Health and Disease

Figure 3

From: Uptake of the glycosphingolipid sulfatide in the gastrointestinal tract and pancreas in vivo and in isolated islets of Langerhans

Figure 3

Sulfatide in pancreas of ob/ob mice. Thin layer chromatography of sulfatide in whole pancreas isolated from ob/ob mice given C16:0 sulfatide. The extraction and lipid isolation procedure as well as the TLC-ELISA method are described under "Methods". Lipids corresponding to approximately 3–4 mg tissue protein were applied (one representative plate is shown). Lane 1, seminolipid (120 pmol); lane 2, sulfated lactosylceramide (20 pmol); lane 3, sulfatide (40 pmol); lane 4, C16:0 sulfatide standard (25 pmol); lane 5, 6 hours after administration; lane 6, 24 hours after administration and lane 7, sulfatide isolated from untreated ob/ob mouse.

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