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Figure 4 | Lipids in Health and Disease

Figure 4

From: Synthesis of the oxysterol, 24(S), 25-epoxycholesterol, parallels cholesterol production and may protect against cellular accumulation of newly-synthesized cholesterol

Figure 4

Endogenously-produced 24,25EC correlates with cholesterol synthesis when macrophages are cholesterol-loaded. (A) THP-1 monocytes differentiated into macrophages for 3 d in 50 ng/μl of PMA. THP-1 macrophages were loaded with acetylated LDL for 24 h whilst labelling with [1-14C]-acetate. Neutral lipid extracts were separated by thin-layer chromatography and bands corresponding to authentic cholesterol and 24,25EC were visualized by phosphorimager and quantified by densitometry. Neutral lipid extracts were separated by thin-layer chromatography and bands corresponding to authentic cholesterol and 24,25EC were visualized by phosphorimager and quantified by densitometry. Equations for the lines of best fit (B) y = 0.88x; R2 = 0.97; P < 0.0001. Values are pooled from 2 separate experiments set relative to the vehicle-treated control condition which was assigned an arbitrary value of 1.

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Lipids in Health and Disease

ISSN: 1476-511X