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Table 3 Fatty Acid Composition of Rat Soleus After Dietary Supplementation with 22:6n-3 and 22:5n-6*

From: Docosahexaenoic acid and n-6 docosapentaenoic acid supplementation alter rat skeletal muscle fatty acid composition

   Artificially reared
  Dam reared (n = 7) AR-LA (n = 7) AR-DPAn-6 (n = 7) AR-DHA+DPAn-6 (n = 8) AR-DHA (n = 7)
Fatty acids (weight % of total fatty acids)
12:0 6.3 ± 2.2a 2.55 ± 0.46b 2.53 ± 0.45b 2.55 ± 0.70b 2.63 ± 0.73b
14:0 5.1 ± 1.1a 3.02 ± 0.27b 3.03 ± 0.25b 3.12 ± 0.40b 3.07 ± 0.51b
16:0 DMA 0.93 ± 0.42 0.75 ± 0.25 0.77 ± 0.37 0.66 ± 0.25 0.67 ± 0.36
16:0 20.1 ± 2.0 20.6 ± 1.6 20.8 ± 0.9 21.6 ± 1.0 20.3 ± 0.6
18:0 DMA 0.54 ± 0.27 0.26 ± 0.12 0.30 ± 0.17 0.28 ± 0.15 0.27 ± 0.15
18:0 8.4 ± 2.2 6.7 ± 1.7 6.6 ± 1.7 5.6 ± 1.4 6.4 ± 2.6
20:0 0.10 ± 0.02a 0.05 ± 0.02b 0.05 ± 0.01b 0.05 ± 0.02b 0.06 ± 0.03b
22:0 0.11 ± 0.08 0.04 ± 0.02 0.05 ± 0.02 0.05 ± 0.05 0.05 ± 0.03
24:0 0.15 ± 0.09a 0.06 ± 0.02b 0.07 ± 0.03ab 0.08 ± 0.06ab 0.09 ± 0.05ab
Total saturates 41.6 ± 0.8a 34.0 ± 2.1b 34.2 ± 2.4b 34.0 ± 1.6b 33.5 ± 2.1b
16:1 n-7 7.3 ± 1.6 7.8 ± 1.4 7.6 ± 1.0 8.3 ± 1.8 7.5 ± 2.3
18:1 DMA 0.08 ± 0.06 0.07 ± 0.02 0.08 ± 0.04 0.10 ± 0.11 0.08 ± 0.05
18:1 n-9 17.3 ± 2.5a 31.2 ± 3.7b 31.6 ± 4.2b 32.8 ± 3.8b 32.1 ± 5.5b
18:1 n-7 3.93 ± 0.36 4.55 ± 0.61 4.40 ± 0.50 4.27 ± 0.45 4.38 ± 0.33
20:1 n-9 0.09 ± 0.02a 0.19 ± 0.03b 0.18 ± 0.05b 0.20 ± 0.04b 0.20 ± 0.05b
24:1 n-9 0.13 ± 0.04 0.12 ± 0.04 0.11 ± 0.04 0.12 ± 0.05 0.14 ± 0.07
Total monounsaturates 28.8 ± 4.2a 44.0 ± 5.2b 44.0 ± 5.1b 45.8 ± 5.3b 44.4 ± 7.9b
18:2 n-6 13.1 ± 1.9a 8.4 ± 1.0b 8.24 ± 0.77b 7.82 ± 0.52b 8.4 ± 1.2b
18:3 n-6 0.03 ± 0.01a 0.03 ± 0.01b 0.024 ± 0.004b 0.022 ± 0.004ab 0.022 ± 0.004ab
20:2 n-6 0.06 ± 0.01a 0.04 ± 0.01b 0.04 ± 0.01b 0.04 ± 0.01b 0.05 ± 0.01ab
20:3 n-6 0.27 ± 0.06 0.22 ± 0.06 0.23 ± 0.07 0.20 ± 0.05 0.20 ± 0.07
20:4 n-6 5.8 ± 1.7 6.2 ± 1.8 5.4 ± 1.7 4.2 ± 1.4 4.9 ± 2.4
22:4 n-6 0.40 ± 0.09ab 0.56 ± 0.14a 0.41 ± 0.11ab 0.27 ± 0.03b 0.31 ± 0.14b
22:5 n-6 0.27 ± 0.10a 2.26 ± 0.73b 3.03 ± 0.99b 0.78 ± 0.29a 0.56 ± 0.32a
Total n-6 PUFA 19.9 ± 3.5a 17.7 ± 3.5ab 17.3 ± 3.2ab 13.3 ± 1.9b 14.4 ± 3.7b
18:3 n-3 0.78 ± 0.23a 0.03 ± 0.01b 0.023 ± 0.003b 0.03 ± 0.01b 0.04 ± 0.04b
20:5 n-3 0.08 ± 0.02a - - 0.02 ± 0.01b 0.025 ± 0.003b
22:5 n-3 0.59 ± 0.19a 0.04 ± 0.02b 0.04 ± 0.01b 0.06 ± 0.03b 0.08 ± 0.03b
22:6 n-3 4.3 ± 1.7a 0.32 ± 0.14b 0.44 ± 0.32b 3.2 ± 2.0a 3.7 ± 2.1a
Total n-3 PUFA 5.7 ± 1.7a 0.40 ± 0.16b 0.50 ± 0.32b 3.3 ± 2.0c 3.8 ± 2.1ac
Total PUFA 25.6 ± 4.5a 18.1 ± 3.7b 17.8 ± 3.4b 16.6 ± 3.9b 18.3 ± 5.7b
Total HUFA 11.7 ± 3.6 9.6 ± 2.8 9.5 ± 3.0 8.7 ± 3.6 9.8 ± 5.0
% of n-3 HUFA in HUFA 41.3 ± 6.3a 3.8 ± 0.6b 4.9 ± 2.7b 35.0 ± 6.9a 38.2 ± 3.12a
Total FA (mg/g) 26.1 ± 8.5 34.5 ± 10.6 37.4 ± 15.6 43.2 ± 17.2 40.0 ± 19.5
  1. *Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds).