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Table 4 Fatty Acid Composition of Rat Red Gastrocnemius After Dietary Supplementation with 22:6n-3 and 22:5n-6*

From: Docosahexaenoic acid and n-6 docosapentaenoic acid supplementation alter rat skeletal muscle fatty acid composition

   Artificially reared
  Dam reared (n = 8) AR-LA (n = 7) AR-DPA (n = 7) AR-DHA+DPA (n = 8) AR-DHA (n = 7)
Fatty acids (weight % of total fatty acids)
12:0 2.10 ± 0.84a 0.96 ± 0.53b 1.07 ± 0.76ab 1.00 ± 0.88b 0.84 ± 0.41b
14:0 2.44 ± 0.54a 1.62 ± 0.55ab 1.75 ± 0.69ab 1.60 ± 0.69ab 1.48 ± 0.48b
16:0 DMA 1.86 ± 0.44 1.37 ± 0.41 1.41 ± 0.46 1.71 ± 0.64 1.89 ± 0.62
16:0 22.1 ± 1.1a 22.5 ± 1.1ab 23.8 ± 1.5b 22.7 ± 0.8ab 22.2 ± 1.1ab
18:0 DMA 0.85 ± 0.26a 0.44 ± 0.19b 0.49 ± 0.16ab 0.57 ± 0.29ab 0.69 ± 0.29ab
18:0 11.9 ± 0.8 10.7 ± 2.1 9.9 ± 2.5 10.6 ± 2.3 10.5 ± 1.4
20:0 0.08 ± 0.02a 0.05 ± 0.01b 0.05 ± 0.02b 0.06 ± 0.01b 0.06 ± 0.01ab
22:0 0.08 ± 0.03 0.06 ± 0.01 0.05 ± 0.02 0.06 ± 0.02 0.08 ± 0.03
24:0 0.17 ± 0.02a 0.10 ± 0.02bc 0.09 ± 0.03b 0.13 ± 0.02cd 0.16 ± 0.03ad
Total saturates 41.6 ± 1.0a 37.8 ± 1.7b 38.6 ± 1.0b 38.4 ± 1.6b 37.9 ± 1.6b
16:1 n-7 3.44 ± 0.63 3.7 ± 1.8 4.1 ± 2.0 3.2 ± 1.2 3.19 ± 0.86
18:1 DMA 0.20 ± 0.06a 0.23 ± 0.09ab 0.24 ± 0.12ab 0.33 ± 0.14ab 0.39 ± 0.14b
18:1 n-9 10.6 ± 1.7a 18.6 ± 5.7ab 19.6 ± 7.1b 17.6 ± 7.0ab 17.7 ± 4.9ab
18:1 n-7 3.34 ± 0.19 3.70 ± 0.55 3.59 ± 0.30 3.70 ± 0.39 3.84 ± 0.40
20:1 n-9 0.07 ± 0.02a 0.13 ± 0.03b 0.14 ± 0.04b 0.12 ± 0.05b 0.11 ± 0.03ab
24:1 n-9 0.16 ± 0.04 0.15 ± 0.09 0.13 ± 0.05 0.18 ± 0.07 0.17 ± 0.03
Total monounsaturates 17.9 ± 2.3 26.5 ± 7.7 27.8 ± 9.1 25.2 ± 8.3 25.4 ± 5.6
18:2 n-6 14.5 ± 1.5a 12.1 ± 1.7b 10.3 ± 1.4b 10.2 ± 0.9b 10.3 ± 1.0b
18:3 n-6 0.03 ± 0.01ab 0.04 ± 0.01a 0.03 ± 0.01ab 0.03 ± 0.01b 0.03 ± 0.01ab
20:2 n-6 0.08 ± 0.05a 0.04 ± 0.01b 0.04 ± 0.01b 0.04 ± 0.01b 0.04 ± 0.01ab
20:3 n-6 0.43 ± 0.05 0.46 ± 0.12 0.43 ± 0.11 0.46 ± 0.15 0.42 ± 0.08
20:4 n-6 8.9 ± 1.0 11.8 ± 3.1 9.8 ± 3.8 9.2 ± 2.7 9.1 ± 1.5
22:4 n-6 0.38 ± 0.04a 0.92 ± 0.24b 0.62 ± 0.23c 0.39 ± 0.08a 0.37 ± 0.07a
22:5 n-6 0.53 ± 0.07a 5.9 ± 1.9b 8.1 ± 2.8b 1.97 ± 0.57a 1.30 ± 0.43a
Total n-6 PUFA 24.8 ± 2.2abc 31.3 ± 6.3b 29.3 ± 8.0bc 22.3 ± 3.8ac 21.6 ± 2.5a
18:3 n-3 0.35 ± 0.07a 0.02 ± 0.01b 0.02 ± 0.01b 0.03 ± 0.01b 0.02 ± 0.01b
20:5 n-3 0.13 ± 0.03a - - 0.03 ± 0.01b 0.04 ± 0.02b
22:5 n-3 1.18 ± 0.11a 0.11 ± 0.03b 0.09 ± 0.03b 0.17 ± 0.05b 0.19 ± 0.06b
22:6 n-3 11.6 ± 1.0a 0.89 ± 0.25b 1.14 ± 0.50b 11.0 ± 3.7a 12.2 ± 2.3a
Total n-3 PUFA 13.2 ± 1.1a 1.02 ± 0.27b 1.24 ± 0.51b 11.2 ± 3.7a 12.4 ± 2.4a
Total PUFA 38.1 ± 2.7 32.3 ± 6.5 30.5 ± 8.2 33.5 ± 7.3 34.0 ± 4.7
Total HUFA 23.1 ± 2.0 20.1 ± 5.4 20.2 ± 7.2 23.2 ± 4.2 23.6 ± 4.2
% of n-3 HUFA in HUFA 55.8 ± 1.8a 5.02 ± 0.30b 6.5 ± 2.8b 47.7 ± 3.3c 52.4 ± 3.0a
Total FA (mg/g) 13.5 ± 1.7 16.4 ± 6.5 16.7 ± 6.7 17.2 ± 8.1 14.3 ± 2.9
  1. *Values are mean ± SD. Values in a row not sharing a roman superscript are significantly different at P < 0.05 by Tukey's honestly significantly difference test after a significant F-value (P < 0.05) by one-way ANOVA. AR-LA, 18:2n-6 artificial reared diet; AR-DPAn-6, 22:5n-6 artificial reared diet; AR-DHA+DPAn-6, 22:6n-3 + 22:5n-6 artificial reared diet; AR-DHA, 22:6n-3 artificial reared diet; DMA, dimethyl acetal; PUFA, polyunsaturated fatty acids; HUFA, highly unsaturated fatty acids (≥ 20 carbons, ≥ 3 carbon-carbon double bonds).