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Table 1 Summary of validation results

From: Plasma lyso-phosphatidylcholine concentration is decreased in cancer patients with weight loss and activated inflammatory status

analyte:

lyso-phosphatidylcholine

sample:

75 μl human plasma

selectivity:

given

calibration range:

60 – 480 μM

analytical function:

quadratic equation (peak area)

linearity:

correlation coefficient r ≥ 0.991

precision and accuracy

calibration:

inter day accuracy ± 6.7% and precision ± 5.5%

QC samples precision:

intra-assay:

± 11%

inter-assay:

± 19%

LOQ

60 μM (inter day accuracy ± 4.7% and precision ± 5.1%)

LOD

< 40 μM

Recovery from plasma

79% ± 2%