Table 1 Amplification primers for GPIHBP1 coding sequence
exon | primer sequence | annealing temperature (°C) | fragment size (base pairs) |
|---|---|---|---|
1 | 5'-CCT TCA TCC CAC TTA CCG CAG C | 60 | 276 |
5'-GCC AGC TTC CAT CCA TGC TGC | |||
2 | 5'-ATG CTT GCC CAG AGC AGG TGT C | 60 | 282 |
5'-GCC TGC TGG CTT CCA TCA CAC | |||
3 | 5'-AGG CTA GGC TTT GGG AGC ACA G | 60 | 307 |
5'-GTCTCTGAGGTGGCTCTGCAG | |||
4 | 5'-CTG CAG AGC CAC CTC AGA GAC | 60 | 447 |
5'-CTG GAT CGC CCA AGA CAC TCC |