Figure 4

Effects of the flavonoids on ceramide and sphingomyeline contents in the normal and ethanol-treated hepatocytes. The cells (10⁶/ml) were incubated at 37°C in Eagle medium containing 70 mM ethanol for 4 h. Control cells were incubated without any additions for 4 h. In some cases, the chamiloflan (500 μg/ml) or AP7Glu (30 μM) were added to the culture medium together with the ethanol addition and cells incubated for 4 h. The ethanol concentration in the flavonoid-treated cell suspension was 70 mM. Lipid contents in the cells were determined as described in the "Materials and Methods". Results are mean ± S.E. of six experiments performed in duplicate. * P < 0.05, ethanol-treated vs. control; ** P < 0.05, ethanol+flavonoid-treated vs. ethanol-treated.