Effects of the flavonoids on ceramide, sphingomyelin and phosphatidylcholine release from the hepatocytes pre-treated by the CCl
. The cells (106/ml) were incubated at 37°C in Eagle medium containing 30 μM ethanol for 2 h followed by 40 mM CCl4treatment for 2 h. Chamiloflan (500 μg/ml) was added to the culture medium 2 h prior 40 mM CCl4 addition and cells incubated for 4 h. Control cells were incubated in the media containing 30 μM ethanol for 4 h. Lipid contents in the culture medium were determined as described in the "Materials and Methods". Results are mean ± S.E. of six experiments performed in duplicate. * P < 0.05, CCl4-treated vs. control; ** P < 0.05, CCl4+flavonoid-treated vs. CCl4-treated.