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Table 1 Composition of the control diet and diet enriched with trans fatty acids according to AIN-93

From: Hydrogenated fat diet intake during pregnancy and lactation modifies the PAI-1 gene expression in white adipose tissue of offspring in adult life

 

Diet (g/100 g)

Ingredient

Control

Trans

Casein*

20 (14)

20

L-cystine†

0.3 (0.18)

0.3

Cornstarch†

62 (71.1)

62

Soybean oil‡

8 (5)

1

Hydrogenated vegetable fat$

-

7

Butylhydroquinone†

0.0014 (0.0008)

0.0014 (0.0008)

Mineral mixture§

3.5

3.5

Vitamin mixture#

1.0

1.0

Cellulose†

5.0

5.0

Choline bitartrate†

0.25

0.25

Energy (kcal/g)

4.0 (3.8)

4.0 (3.8)

  1. The first number refers to the growth diet (AIN-93G) and the number in parentheses refers to maintenance diet (AIN-93M) when its composition differed from that of the growth diet. *Casein was obtained from CoperLab, São Paulo, Brazil. †L-cystine, cornstarch, butylhydroquinone, cellulose and choline bitartrate were obtained from Viafarma, São Paulo, Brazil. ‡Oil was supplied from soybean (Lisa/Ind. Brazil). $Hydrogenated vegetabke fat was supplied from Unilever, São Paulo, Brazil. £Mineral mix 9mg/kg diet): calcium, 5000; phosphorus, 1561; potassium, 3600; sodium, 1019; chloride, 1571; sulfur, 300; magnesium, 507; iron, 35; copper, 6.0; manganese, 10.0; zinc, 30.0; chromium, 1.0; iodine 0.2; selenium, 0.15; fluoride, 1.00; boron, 0.50; molybdenum, 0.15; silicon, 5.0; nickel, 0.5; lithium, 0.1; vanadium, 0.1 (AIN-93G mineral mix DYETS 210025, Dyets Inc., Bethlehem, PA, USA). #Vitamin mix (mg/kg diet): thiamin HCL, 6.0, riboflavin, 6.0; pyridoxine HCL 7.0; niacin, 30.0; calcium pantothenate, 16.0; folic acid, 2.0; biotin, 0.2; vitamin B12, 25.0; vitamin A palmitate 4000 IU; vitamin E acetate, 75; vitamin D3, 1000 IU; vitamin KI, 0.75. (AIN-93G, vitamin mix, DYETS 310025, Dyets Inc., Bethlehem, PA, USA)