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Table 1 Composition of the control diet and diet enriched with trans fatty acids according to AIN-93

From: Hydrogenated fat diet intake during pregnancy and lactation modifies the PAI-1 gene expression in white adipose tissue of offspring in adult life

  Diet (g/100 g)
Ingredient Control Trans
Casein* 20 (14) 20
L-cystine 0.3 (0.18) 0.3
Cornstarch 62 (71.1) 62
Soybean oil 8 (5) 1
Hydrogenated vegetable fat$ - 7
Butylhydroquinone 0.0014 (0.0008) 0.0014 (0.0008)
Mineral mixture§ 3.5 3.5
Vitamin mixture# 1.0 1.0
Cellulose 5.0 5.0
Choline bitartrate 0.25 0.25
Energy (kcal/g) 4.0 (3.8) 4.0 (3.8)
  1. The first number refers to the growth diet (AIN-93G) and the number in parentheses refers to maintenance diet (AIN-93M) when its composition differed from that of the growth diet. *Casein was obtained from CoperLab, São Paulo, Brazil. L-cystine, cornstarch, butylhydroquinone, cellulose and choline bitartrate were obtained from Viafarma, São Paulo, Brazil. Oil was supplied from soybean (Lisa/Ind. Brazil). $Hydrogenated vegetabke fat was supplied from Unilever, São Paulo, Brazil. £Mineral mix 9mg/kg diet): calcium, 5000; phosphorus, 1561; potassium, 3600; sodium, 1019; chloride, 1571; sulfur, 300; magnesium, 507; iron, 35; copper, 6.0; manganese, 10.0; zinc, 30.0; chromium, 1.0; iodine 0.2; selenium, 0.15; fluoride, 1.00; boron, 0.50; molybdenum, 0.15; silicon, 5.0; nickel, 0.5; lithium, 0.1; vanadium, 0.1 (AIN-93G mineral mix DYETS 210025, Dyets Inc., Bethlehem, PA, USA). #Vitamin mix (mg/kg diet): thiamin HCL, 6.0, riboflavin, 6.0; pyridoxine HCL 7.0; niacin, 30.0; calcium pantothenate, 16.0; folic acid, 2.0; biotin, 0.2; vitamin B12, 25.0; vitamin A palmitate 4000 IU; vitamin E acetate, 75; vitamin D3, 1000 IU; vitamin KI, 0.75. (AIN-93G, vitamin mix, DYETS 310025, Dyets Inc., Bethlehem, PA, USA)