Figure 2From: Plasma PCSK9 levels are significantly modified by statins and fibrates in humansEffect of increasing concentrations of fenofibrate on PCSK9 and LDLR protein expression in HepG2 cells. Cells were grown in DMEM + 10% FBS and supplemented with increasing concentrations of fenofibrate for 24 hours at 37°C. 50 μg of total cell lysates (A and C) and 100 μl cell media (B) were analyzed by immunoblotting (IB), or immunoprecipitation (IP) followed by IB, respectively, as described in Methods. A and B were IB with anti-hPCSK9 Ab and C with anti-hLDLR Ab. The protein signals were quantified by densitometry using Syngene Chemigenius 2XE imager and Gene Tools software. All values were made relative to values from untreated cells set as 1 and presented as mean ± SEM (n = 3).Back to article page