Figure 2
From: Lipoprotein binding preference of CD36 is altered by filipin treatment
Effect of specific antibodies against human CD36 and unlabeled oxLDL on the binding of Dil-labeled OxLDL to CHO-CD36 cells. CHO-CD36 cells were first incubated with the anti-CD36 antibody FA6 (panel A) or mouse serum IgG (Panel B) or unlabeled oxLDL (panel C) and then 10 μg/ml of Dil-labeled OxLDL was added to incubate for a further 2 – 4 hours. The concentrations of anti-CD36 antibody in panel A and of mouse IgG serum in panel B from top to bottom are: 0, 2, 4, 6, 8 and 10 μg/ml respectively. The concentrations of unlabelled OxLDL in panel C from top to bottom are: 20, 40, 100, 200, and 400 μg/ml. Lipoprotein binding to cells (expressed as mean relative fluoresence) was determined by flow cytometry.