Figure 3
From: Lipoprotein binding preference of CD36 is altered by filipin treatment
Effects of filipin on Dil-lipoprotein binding to CHO-CD36 cells. Panel A: CHO-CD36 cells were pre-treated with 10 μg/ml of filipin at 37°C for 30 minutes and then they were exposed to 10 μg/ml of Dil-lipoprotein at 37°C for 4 hours. The cells were fixed with 4% formaldehyde and viewed under fluorescent microscope. Panel B: Cells were treated same as in A and were then assayed for the presence of Dil by flow cytometry. The data in B represents an average result of three independent experiments.