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Figure 2 | Lipids in Health and Disease

Figure 2

From: Enhanced production of nitric oxide, reactive oxygen species, and pro-inflammatory cytokines in very long chain saturated fatty acid-accumulated macrophages

Figure 2

NO production and iNOS gene expression in macrophages. (A) Cells were incubated with LPS (1, 10, 100 ng/ml) plus IFN-γ (2 ng/ml) for 24 h at 37°C. Control contained neither LPS nor IFN-γ. After incubation, culture supernatants were collected and NO was measured with Griess reagent. (B) Cells were incubated with LPS (10 ng/ml) plus IFN-γ (2 ng/ml) for indicated period at 37°C. After incubation, culture supernatants were collected and NO was measured with Griess reagent. Results are expressed as mean ± SD, n = 6. (C) Cells were incubated with LPS (10 ng/ml) plus IFN-γ (2 ng/ml) for 18 h at 37°C. After incubation, total RNA was extracted and iNOS gene expression was analyzed by real-time RT-PCR. Results are mean-fold increase in iNOS mRNA levels of MPMs from ALDP-deficient mice compared with those of wild-type mice and expressed as mean ± SD, n = 6. * P < 0.05 for comparison with wild-type.

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