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Figure 1 | Lipids in Health and Disease

Figure 1

From: Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells

Figure 1

Effect of polyunsaturated fatty acids and PMA on the production of MMP-9. MonoMac 6 cells (0.8 × 106/ml in serum-free medium X-Vivo 15) were stimulated with 1 or 10 μM arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosapentaenoic acid (DPA) without (1A) or in the presence of 1.3 nM PMA (1B). The culture media were harvested after 24 h incubation and analysed for MMP-9 by gelatin zymography. The media of PMA-treated cells were diluted (1:10) to preserve the linearity of response in image analysis.

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