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Figure 2 | Lipids in Health and Disease

Figure 2

From: Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells

Figure 2

Dose- and time-dependent stimulation of MMP-9 secretion by arachidonic acid. 2A. MonoMac 6 cells (0.8 × 106 cells/ml in X-Vivo 15) were treated for 24 h with increasing concentrations of arachidonic acid. The media were collected and analysed for MMP-9 by zymography. Data are shown as means ± SEM of 3 to 4 independent experiments. The increase of MMP-9 secretion was statistically significant from 2.5 μM AA and upwards. 2B. MonoMac 6 cells (0.8 × 106 cells/ml in X-Vivo 15) were pre-exposed to 10 μM arachidonic acid for 1, 2, 4 or 6 hours where after the culture medium was changed to X-Vivo without arachidonic acid for 24 h. The media were analysed for MMP-9 by zymography. Results are shown as means ± SEM of three independent experiments. A statistically significant increase in MMP-9 secretion was evident after 1 h preincubation in comparison with cells incubated in the presence of vehicle only.

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Lipids in Health and Disease

ISSN: 1476-511X