Skip to main content
Figure 3 | Lipids in Health and Disease

Figure 3

From: Oleic acid and peanut oil high in oleic acid reverse the inhibitory effect of insulin production of the inflammatory cytokine TNF-α both in vitro and in vivo systems

Figure 3

TNF-α or oleic acid treatment has no apoptotic inducing effect on INS-1 cells. Cells were pre-treated with low (1 μM) and high (10 μM) oleic acid, low (100 pg/mL) and high (1,000 pg/mL) TNF-α or pre-treated for 2 hours with oleic acid (10 μM) and subsequently treated with TNF-α (100 pg/mL). Twenty-four hours later the apoptotic status of cells was assessed using flow cytometry (Annexin V/PI staining). Results are expressed as percent of apoptotic/necrotic cells (upper right and lower right quadrant). Shown is one representative experiment performed in duplicate out of three independent experiments.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X