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Figure 4 | Lipids in Health and Disease

Figure 4

From: Oleic acid and peanut oil high in oleic acid reverse the inhibitory effect of insulin production of the inflammatory cytokine TNF-α both in vitro and in vivo systems

Figure 4

(A) Oleic acid treatment does not increase intracellular cAMP in INS-1 cells. Cells were treated with oleic acid at 10 μM, 5 μM, 1 μM and 0.1 μM for 15 min. Cellular homogenates were subjected to cAMP assay. Shown is one representative experiment with triplicate values out of two independent experiments. No significant difference was observed between controls and treatments. (B) Oleic acid induces translocation of PPAR-γ in INS-1 cells. Cells were treated with oleic acid at 10 μM, 5 μM, 1 μM, TNF-α (100 pg/mL) or combination of TNF-α and oleic acid (10 μM) for 18–24 hrs. Nuclear extracts were subjected to PPAR-γ detection assay. Shown is one representative experiment with triplicate values out of three independent experiments. *, p < 0.05 for cells treated with oleic acid (10 μM) compared to control and combination of TNF-α (100 pg) with oleic acid (10 μM) compared to cells treated with TNF-α alone.

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