Figure 3

(A). Fractionation of lip lipids by Silica cartridge and detection of docosahexaenoic acid in Fraction I. (left top) total ion chromatography (TIC) pattern of standard fish oil fatty acid methyl esters. (left second top) TIC of total fatty acid methyl esters from total lipid extract of lip surface obtained at 11:30 clock time. (left third top) TIC of total fatty acid methyl esters from lipid extract obtained at 15:30 clock time. (left bottom) TIC of Fraction I fatty acid methyl esters. The peak of DHA methyl ester was clearly observed as shown in the inserted figure (enlarged chromatogram). (right bottom) TIC of Fraction II fatty acid methyl esters and almost no DHA peak was observed. Fig. 3(B)(right top) TLC patterns of lipids isolated in Fraction I, II, and III by silica gel cartridge. Squalene was applied in the sq lane. Developmental solvent was the same as Fig. 2.