Figure 4From: Metabolism of fatty acids and lipid hydroperoxides in human body monitoring with Fourier transform Infrared SpectroscopyFTIR spectrum of lip surface and detection of polyunsaturated fatty acids and surface ultrastructures. (A) FTIR spectrum of lip surface; The inserted figure shows the second derivative spectra for cis-alkene absorption area measured at 9:30, 11:30, and 13:30 clock time. FTIR spectra was measured in 8 cm-1 resolution and 48 accumulations within 40 seconds. (B) Tape-stripped lip surfaces stained by Alcian blue. (left) labial vermilion surface: (middle)enlarged spot of a part of left figure: (right) labial vermilion-skin border surface. The left and right pictures were taken by stereomicroscope (low mag), and the middle was by inverted microscope (Olympus, Japan) with hemocytometer cover slip (Cellocate, Eppendorf) as a field width indicator. The spot size was 20~40 μm in diameter. (C) 2nd derivative spectra in cis-alkene area for lips of 23-yrs old subjects with DHA and control diets. Docosahexaenoic acid shows typical cis-alkene IR absorption at 3014 cm-1, and arachidonic acid shows at 3012 cm-1.Back to article page