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Figure 4 | Lipids in Health and Disease

Figure 4

From: Metabolism of fatty acids and lipid hydroperoxides in human body monitoring with Fourier transform Infrared Spectroscopy

Figure 4

FTIR spectrum of lip surface and detection of polyunsaturated fatty acids and surface ultrastructures. (A) FTIR spectrum of lip surface; The inserted figure shows the second derivative spectra for cis-alkene absorption area measured at 9:30, 11:30, and 13:30 clock time. FTIR spectra was measured in 8 cm-1 resolution and 48 accumulations within 40 seconds. (B) Tape-stripped lip surfaces stained by Alcian blue. (left) labial vermilion surface: (middle)enlarged spot of a part of left figure: (right) labial vermilion-skin border surface. The left and right pictures were taken by stereomicroscope (low mag), and the middle was by inverted microscope (Olympus, Japan) with hemocytometer cover slip (Cellocate, Eppendorf) as a field width indicator. The spot size was 20~40 μm in diameter. (C) 2nd derivative spectra in cis-alkene area for lips of 23-yrs old subjects with DHA and control diets. Docosahexaenoic acid shows typical cis-alkene IR absorption at 3014 cm-1, and arachidonic acid shows at 3012 cm-1.

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