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Figure 3 | Lipids in Health and Disease

Figure 3

From: Novel point mutations attenuate autotaxin activity

Figure 3

Kinetic analysis. Hydrolyses were detected as described in Methods. A. LPC hydrolysis. Reactions contained 30 ng ATX protein. B. FS-3 hydrolysis. Reactions contained 30 ng (WT) or 360 ng (H226Q) ATX protein. C. pNpTMP hydrolysis. Reactions contained 30 ng (WT) or 360 ng (H226Q) ATX protein. For all reactions, product formation was linear (R2 = 0.99) between 30 minutes and 3 hours. Data are expressed as relative reaction rates [RFU (or A405)/time] and plotted against substrate concentrations. WT: wild type; 420: H420Q; 434: H434Q; 226: H226Q.

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