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Table 1 Kinetic Parameters

From: Novel point mutations attenuate autotaxin activity

  

Vmax

  
 

WT

H226Q

H434Q

H420Q

LPC 16:0 a

306 ± 60

149 ± 63

111 ± 26

359 ± 63

LPC 18:0 a

129 ± 56

76 ± 37

NS

149 ± 49

LPC 18:1 a

589 ± 96

267 ± 59 *

107 ± 13 *

472 ± 143

FS-3 a

142 ± 4

26 ± 1

NS

ND

pNpTMP b

21 ± 0.8

7 ± 2.0

NS

ND

  

Km

  
 

WT

H226Q

H434Q

H420Q

LPC 16:0 c

66 ± 11

126 ± 9 *

120 ± 15 *

94 ± 8

LPC 18:0 c

89 ± 9

270 ± 152

NS

115 ± 31

LPC 18:1 c

73 ± 8

126 ± 20

105 ± 21

69 ± 18

FS-3 c

2.6 ± 0.3

19 ± 1.1

NS

ND

pNpTMP d

0.7 ± 0.1

4.8 ± 2.2

NS

ND

  1. a: Units are RFU/min./μg ATX protein ± SEM.
  2. b: Units are A405 × 10000/min./μg ATX protein ± SEM.
  3. c: Units are μM ± SEM, d: Units are mM ± SEM.
  4. *: p < .05 vs WT (one-way ANOVA with Dunnett's post test).
  5. NS: data not significant, ND: not determined.
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Lipids in Health and Disease

ISSN: 1476-511X