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Table 1 Kinetic Parameters

From: Novel point mutations attenuate autotaxin activity

   Vmax   
  WT H226Q H434Q H420Q
LPC 16:0 a 306 ± 60 149 ± 63 111 ± 26 359 ± 63
LPC 18:0 a 129 ± 56 76 ± 37 NS 149 ± 49
LPC 18:1 a 589 ± 96 267 ± 59 * 107 ± 13 * 472 ± 143
FS-3 a 142 ± 4 26 ± 1 NS ND
pNpTMP b 21 ± 0.8 7 ± 2.0 NS ND
   Km   
  WT H226Q H434Q H420Q
LPC 16:0 c 66 ± 11 126 ± 9 * 120 ± 15 * 94 ± 8
LPC 18:0 c 89 ± 9 270 ± 152 NS 115 ± 31
LPC 18:1 c 73 ± 8 126 ± 20 105 ± 21 69 ± 18
FS-3 c 2.6 ± 0.3 19 ± 1.1 NS ND
pNpTMP d 0.7 ± 0.1 4.8 ± 2.2 NS ND
  1. a: Units are RFU/min./μg ATX protein ± SEM.
  2. b: Units are A405 × 10000/min./μg ATX protein ± SEM.
  3. c: Units are μM ± SEM, d: Units are mM ± SEM.
  4. *: p < .05 vs WT (one-way ANOVA with Dunnett's post test).
  5. NS: data not significant, ND: not determined.