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Figure 5 | Lipids in Health and Disease

Figure 5

From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

Figure 5

Bands of detergent-resistant membrane fractions in sucrose gradients after ultracentrifugation. Boar sperm cells were incubated with detergents for 30 min at 4°C, membrane fractions were mechanically detached and separated from the remaining cell pellet by centrifugation. The supernatant was transferred under a continuous sucrose gradient for subsequent flotation. The obtained density gradient is additionally shown at the right of the figure. For further details see the Materials and Methods section.

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Lipids in Health and Disease

ISSN: 1476-511X