| |
Release into supernatant after detergent and mechanical treatment
|
Bottom fraction related to the underlayed amount from supernatant
|
Band fractions 1/2 related to the underlayed amount from supernatant
|
|---|
|
Detergent
|
Cholesterol
|
Phospholipid
|
Protein
|
Cholesterol
|
Phospholipid
|
Protein
|
Cholesterol
|
Phospho-lipid
|
Protein
|
| |
[%]
|
[%]
|
[mg/10
9
cells]
|
[%]
|
[%]
|
[%]
|
[%]
|
[%]
|
[%]
|
|
w/o
|
0.0
|
5.2
|
0.49
| |
1.7
|
34.6
| |
49.0
|
13.7
|
|
Pluronic F-127
|
0.0
|
2.3
|
0.68
| |
1.9
|
13.7
| |
10.2
|
0.0
|
|
Sodium cholate
|
79.8
|
79.0
|
3.07
|
4.5
|
5.4
|
20.0
|
36.4
|
68.6
|
29.3
|
|
CHAPS
|
88.5
|
85.7
|
2.57
|
10.0
|
20.0
|
24.6
|
0.8/11.3
|
1.0/19.0
|
0.0/21.0
|
|
Tween 20
|
0.0
|
9.1
|
2.01
| |
4.2
|
15.9
| |
38.3
|
5.8
|
|
Triton X-100
|
50.4
|
68.9
|
2.28
|
8.0
|
8.4
|
18.3
|
11.4/10.5
|
9.8/7.9
|
0.0/4.7
|
|
Brij 96V
|
61.5
|
61.7
|
2.27
|
3.3
|
2.6
|
20.8
|
1.7
|
2.5
|
3.8
|
- The sum of the amounts of cholesterol and phospholipid in the pellet and the supernatant is considered as 100%. Additionally, total amounts of protein released into the supernatant (per 109 cells) and relative amounts of cholesterol, phospholipid and protein in the visible bands and bottom fractions from sucrose gradients after ultracentrifugation are also given. For further details see Materials and Methods section.
