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Table 2 Relative amounts of cholesterol and phospholipid that were released from boar spermatozoa into the supernatant after detergent solubilisation, mechanical treatment and centrifugation

From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

  Release into supernatant after detergent and mechanical treatment Bottom fraction related to the underlayed amount from supernatant Band fractions 1/2 related to the underlayed amount from supernatant
Detergent Cholesterol Phospholipid Protein Cholesterol Phospholipid Protein Cholesterol Phospho-lipid Protein
  [%] [%] [mg/10 9 cells] [%] [%] [%] [%] [%] [%]
w/o 0.0 5.2 0.49   1.7 34.6   49.0 13.7
Pluronic F-127 0.0 2.3 0.68   1.9 13.7   10.2 0.0
Sodium cholate 79.8 79.0 3.07 4.5 5.4 20.0 36.4 68.6 29.3
CHAPS 88.5 85.7 2.57 10.0 20.0 24.6 0.8/11.3 1.0/19.0 0.0/21.0
Tween 20 0.0 9.1 2.01   4.2 15.9   38.3 5.8
Triton X-100 50.4 68.9 2.28 8.0 8.4 18.3 11.4/10.5 9.8/7.9 0.0/4.7
Brij 96V 61.5 61.7 2.27 3.3 2.6 20.8 1.7 2.5 3.8
  1. The sum of the amounts of cholesterol and phospholipid in the pellet and the supernatant is considered as 100%. Additionally, total amounts of protein released into the supernatant (per 109 cells) and relative amounts of cholesterol, phospholipid and protein in the visible bands and bottom fractions from sucrose gradients after ultracentrifugation are also given. For further details see Materials and Methods section.