|
Ether-PE
|
SM
|
Plasm-PE
|
PI
|
Ether-PC
|
PC-Plasm
|
PE:SM:PC
|
---|
Triton X-100, Supernatant
|
24.8
|
29.1
|
14.4
|
---
|
63.0
|
26.9
|
44:32:100
|
Triton X-100, Pellet
|
10.6
|
18.2
|
3.4
|
---
|
22.3
|
13.7
|
39:51:100
|
Triton X-100, Band 1
|
22.2
|
45.2
|
17.2
|
14.8
|
43.0
|
14.9
|
68:78:100
|
Triton X-100, Band 2
|
8.2
|
15.6
|
4.4
|
traces
|
16.3
|
6.1
|
56:70:100
|
Triton X-100, Bottom
|
14.9
|
14.8
|
13.6
|
---
|
36.0
|
23.1
|
48:25:100
|
Sodium cholate, Supernatant
|
21.2
|
51.6
|
9.1
|
---
|
59.8
|
35.8
|
32:54:100
|
Sodium cholate, Pellet
|
8.2
|
traces
|
traces
|
---
|
12.5
|
4.2
|
49:0:100
|
Sodium cholate, Band
|
79.0
|
95.7
|
56.9
|
traces
|
209.4
|
57.5
|
51:36:100
|
Sodium cholate, Bottom
|
8.5
|
13.6
|
3.7
|
---
|
24.1
|
12.6
|
33:16:100
|
CHAPS, Supernatant
|
30.8
|
39.2
|
17.0
|
---
|
66.3
|
32.7
|
48:40:100
|
CHAPS, Pellet
|
traces
|
traces
|
traces
|
---
|
12.1
|
6.5
|
n.d.
|
CHAPS, Band 1
|
traces
|
traces
|
traces
|
---
|
traces
|
traces
|
n.d.
|
CHAPS, Band 2
|
traces
|
traces
|
traces
|
---
|
traces
|
traces
|
n.d.
|
CHAPS, Bottom
|
34.5
|
58.9
|
22.9
|
traces
|
88.9
|
43.0
|
44:45:100
|
- All concentrations were determined by 31P NMR relative to PA (14:0/14:0) that was present in all cases in a final concentration of 32.5 μmol/l (cf. Fig. 6). Please note that all data cannot be given with an estimated accuracy higher than ≤ 15%. This is due to the relatively small concentrations, the low sensitivity of NMR and the partial overlap between the individual species. This particularly concerns the PE species and SM. The last column gives the calculated intensity ratio between PE, SM and PC without further differentiation. PC was arbitrarily set as 100% in all cases. For details see text.