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Table 3 Molar concentrations of different phospholipid species in the indicated fractions

From: The solubilisation of boar sperm membranes by different detergents - a microscopic, MALDI-TOF MS, 31P NMR and PAGE study on membrane lysis, extraction efficiency, lipid and protein composition

  Ether-PE SM Plasm-PE PI Ether-PC PC-Plasm PE:SM:PC
Triton X-100, Supernatant 24.8 29.1 14.4 --- 63.0 26.9 44:32:100
Triton X-100, Pellet 10.6 18.2 3.4 --- 22.3 13.7 39:51:100
Triton X-100, Band 1 22.2 45.2 17.2 14.8 43.0 14.9 68:78:100
Triton X-100, Band 2 8.2 15.6 4.4 traces 16.3 6.1 56:70:100
Triton X-100, Bottom 14.9 14.8 13.6 --- 36.0 23.1 48:25:100
Sodium cholate, Supernatant 21.2 51.6 9.1 --- 59.8 35.8 32:54:100
Sodium cholate, Pellet 8.2 traces traces --- 12.5 4.2 49:0:100
Sodium cholate, Band 79.0 95.7 56.9 traces 209.4 57.5 51:36:100
Sodium cholate, Bottom 8.5 13.6 3.7 --- 24.1 12.6 33:16:100
CHAPS, Supernatant 30.8 39.2 17.0 --- 66.3 32.7 48:40:100
CHAPS, Pellet traces traces traces --- 12.1 6.5 n.d.
CHAPS, Band 1 traces traces traces --- traces traces n.d.
CHAPS, Band 2 traces traces traces --- traces traces n.d.
CHAPS, Bottom 34.5 58.9 22.9 traces 88.9 43.0 44:45:100
  1. All concentrations were determined by 31P NMR relative to PA (14:0/14:0) that was present in all cases in a final concentration of 32.5 μmol/l (cf. Fig. 6). Please note that all data cannot be given with an estimated accuracy higher than ≤ 15%. This is due to the relatively small concentrations, the low sensitivity of NMR and the partial overlap between the individual species. This particularly concerns the PE species and SM. The last column gives the calculated intensity ratio between PE, SM and PC without further differentiation. PC was arbitrarily set as 100% in all cases. For details see text.