ABCG1 BAC Tg mice over-express the ABCG1 transporter in the cortex, hippocampus, and cerebellum, and show normal behaviour. A) Crude membrane fractions were extracted from dissected brain regions and were subjected to Western blot. Blots were probed by polyclonal antibodies recognizing human and murine ABCG1 or Na/K-ATPase as a loading control. ABCG1 protein levels are increased 6-fold in cortex (Cor) and hippocampus (Hipp) and 3-fold in cerebellum (Cer) of ABCG1-BAC-Tg (Tg) mice relative to baseline levels in the equivalent region from wild-type (WT) controls. B) SHRPA primary screen on ABCG1 BAC Tg (ABCG1; n = 11) and wild-type (WT; n = 11) mice. Primary screen involves physiological profiling of each mouse, using a number of test categories and assigning a rating for each mouse in each category. All p values are non-significant when compared to WT animals.