Figure 5
From: Boswellic acid inhibits expression of acid sphingomyelinase in intestinal cells
Western blot showing the effect of AKBA on acid SMase expression. Caco-2 cells were treated with AKBA in different concentrations for 24 h. Fifty micrograms of proteins were subjected to 7.5% SDS-PAGE and transferred to nitrocellulose membrane electrophoretically. The membrane was probed with anti-acid SMase antibody first and then a secondary antibody. The bands were identified by ECL advance reagents (upper panel). In the lower panel, the membrane was stripped and re-probed with anti-β-actin antibody. The results from two separate samples for each concentration group are presented. Similar results were obtained from other two experiments.