Figure 6
From: Boswellic acid inhibits expression of acid sphingomyelinase in intestinal cells
Changes of acid SMase mRNA in Caco-2 cells after AKBA stimulation. Total RNA was extracted from Caco-2 cells treated with AKBA and subjected to qRT-PCR to measure the expression of acid SMase mRNA. GAPDH was used as a reference gene. Ct value and relative levels of mRNA were calculated by the iQ™5 Optical System Software. Similar results were obtained from other two experiments. **P < 0.01.