Table 1 The primer Sequences Used for PCR Amplification
Gene | Genebank Accession No. | Annealing temperature | Primer sequence 5' to 3' |
|---|---|---|---|
Per2 | NM_011066 | 58°C | Forward Primer: CAGACTCATGATGACAGAGG Reverse Primer: GAGATGTACAGGATCTTCCC |
Bmal1 | NM_007489 | 62°C | Forward Primer: CACTGACTACCAAGAAAGTATG Reverse Primer: ATCCATCTGCTGCCCTGAGA |
Clock | NM_007715 | 58°C | Forward Primer: CTTCCTGGTAACGCGAGAAAG Reverse Primer: TCGAATCTCACTAGCATCTGACT |
Cry1 | NM_007771 | 58°C | Forward Primer: CACTGGTTCCGAAAGGGACTC Reverse Primer: CTGAAGCAAAAATCGCCACCT |
Rev-Erbα | NM_145434 | 61°C | Forward Primer: TACATTGGCTCTAGTGGCTCC Reverse Primer: CAGTAGGTGATGGTGGGAAGTA |
PPARα | NM_011144 | 61°C | Forward Primer: TCGGCGAACTATTCGGCTG Reverse Primer: GCACTTGTGAAAACGGCAGT |
RXRα | NM_009024 | 61°C | Forward Primer: CTGCACTCTCCTATCAGCACC Reverse Primer: AGTCCCGAAGCCCAATGTG |
GAPDH | BC_083149 | 62°C | Forward Primer: ACAGCCGCATCTTCTTGTGCAGTA Reverse Primer: GGCCTTGACTGTGCCGTTGAATTT |