Reduced levels of SCD1 accentuate palmitate-induced stress in insulin-producing β-cells

Table 1 Cellular proteins differentially regulated by SCD1 knockdown.

Protein	SCD1 KD
Rho GDP dissociation inhibitor (GDI) α	36% up
Prolyl 4-hydroxylase, β	80% up
GRP94	64% up
Proteasome subunit α type 3	52% up
Proteasome 26 S subunit	75% up
Heterogeneous nuclear ribonucleoprotein H1	46% up
Protein disulfide isomerase associated 3	78% up
Nucleosome assembly protein	136% up
Seryl-aminoacyl-tRNA synthetase	93% up
Nucleolysin TIAR	144% up
Phosphoserine aminotransferase 1	73% up

MIN6 cells were transfected with siRNA targeted towards SCD1 or with nonsense siRNA (mock). After 72 hours culture, cellular proteins were isolated and subjected to 2D-gel electrophoresis. After staining, differentially expressed proteins were cut and identified using peptide mass fingerprint. Differences indicate change induced by reduction in SCD1 levels. Results are means of 4 independent experiments. Significantly different (P < 0.05) expressed proteins in SCD1 KD cells compared with mock transfected cells are shown.

ISSN: 1476-511X