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Figure 5 | Lipids in Health and Disease

Figure 5

From: Sphingolipids: the nexus between Gaucher disease and insulin resistance

Figure 5

Western blot analysis of Akt phosphorylation in a Gaucher cell model. A Gaucher cell phenotype was induced in THP-1 macrophages with conduritol B epoxide as described previously [8]. Cells were stimulated for 5 minutes with 1 μg/ml heparinase (Sigma), extracts prepared and probed for the phosphorylated form of Akt (Ser473) (top panel) and total Akt (bottom panel). Antibodies were purchased from Cell Signaling Technology.

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