Figure 1

Role of CD14 and TLR4 in secretion of IL-1β in response to mmLDL. Human monocytes (1A) and macrophages (1B) were treated with anti-CD14, anti-TLR4, or both antibodies (10 μg/ml) for 1 hour before incubation with mmLDL (50 μg/ml and 70 μg/ml, respectively). Monocytes and macrophages were incubated with irrelevant antibody (10 μg/ml) in the presence or absence of mmLDL (50 μg/ml and 70 μg/ml, respectively). The concentration of IL-1β in culture supernatants was determined by CBA. *p < 0.005.