Figure 4

Role of CD14, TLR4, and TLR2 in secretion of IL-10 in response to mmLDL. Human monocytes (4A) and macrophages (4C) were treated with anti-CD14, anti-TLR4, or both antibodies (10 μg/ml) for 1 hour before incubation with mmLDL (50 μg/ml and 70 μg/ml, respectively). Monocytes (4B) and macrophages (4D) were treated with anti-TLR2 (10 μg/ml) for 1 hour before stimulation with mmLDL (50 μg/ml and 70 μg/ml, respectively). Monocytes and macrophages were incubated with irrelevant antibody (10 μg/ml) in the presence or absence of mmLDL (50 μg/ml and 70 μg/ml, respectively). The concentration of IL-10 in culture supernatants was determined by CBA. *p < 0.005.