Effects of hemin and/or Ad-HO-1 on hepatic HO-1 protein expression in the liver of mice. (A) Immunostaining for HO-1 protein. (B) Effect of hemin and/or Ad-HO-1 on quantitative protein expression of HO-1. The expression of HO-1 was estimated by average area density (areas of positive cells/total areas) (original magnification, × 200). Data are expressed as the mean ± SD (n = 6 per group). *P < 0.001, compared with control; #P < 0.01, compared with MCD feeding mice; $P < 0.01, compared with MCD+ Ad-GFP treated mice.