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Table 1 Primers for real-time quantitative PCR analysis.

From: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

Gene

Product length

Primer sequences

CYP2E1

199 bp

F 5'-AACAGAGACCACCAGCACA-3'

  

R 5'-GGAAGGGACGAGGTTGATGA-3'

Fas

504 bp

F 5'-TGCGATTCTCCTGGCTGTGA-3'

  

R 5'-GGTTCTGCGACATTCGGCTT-3'

FasL

345 bp

F 5'-GAGTTCACCAACCAAAGCCTT-3'

  

R 5'-CAACCTCTTCTCCTCCATTAGC-3'

Bcl-2

100 bp

F 5'-GGATGACTTCTCTCGTCGCTAC-3'

  

R 5'-TGACATCTCCCTGTTGACGCT-3'

Bax

239 bp

F 5'-GGTTGCCCTCTTCTACTTTGC-3'

  

R 5'-TCTTCCAGATGGTGAGCGAG-3'

Cyt-C

157 bp

F 5'-CGGCTGCTGTGATTGTGAAT-3'

  

F 5'-TGTCTTGTGTTTCCCGCCTT-3'

caspase-3

439 bp

F 5'-ACGCAGCCAACCTCAGAGA-3'

  

R 5'-ATGAACCACGACCCGTCCT-3'

caspase-9

171 bp

F 5'-TCCTCTCTTCATCTCCTGCTTAG-3'

  

R 5'-ACTACTCTCTGCTCCTTTGCTG-3'

GAPDH

450 bp

F 5'-ACCACAGTCCATGCCATCAC-3'

  

R 5'-TCCACCACCCTGTTGCTG-3'

  1. Abbreviations: CYP2E1, cytochrome p4502E1; Fas, death receptor; FasL, Fas Ligand; Cyt-C, cytochrome C; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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Lipids in Health and Disease

ISSN: 1476-511X