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Table 2 Effect of Kurozu concentrated liquid (KCL) on 3T3-L1 preadipocyte differentiation and the expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated γ (PPARγ) mRNA during 3T3-L1 preadipocyte differentiation

From: Effects of Kurozu concentrated liquid on adipocyte size in rats

  Phase I (mean ± SE) Phase I & II (mean ± SE)
  Acetic acid KCL Acetic acid KCL
Absorbance 0.139 ± 0.002a 0.124 ± 0.004 0.135 ± 0.001b 0.125 ± 0.001
aP2/β-actin (%) 73.7 ± 0.7b 56.0 ± 2.8 108 ± 1c 32.7 ± 0.5
PPARγ/β-actin (%) 1.74 ± 0.03c 0.943 ± 0.032 1.36 ± 0.04 N.D.
  1. Differentiated cells were stained with Oil Red O and their absorbance was determined at 492 nm. In the determination of mRNA levels, β-actin was used as a loading control. The means and standard errors (SE) are for triplicate experiments. Different superscript letters indicate significant differences at aP < 0.05, bP < 0.01, cP < 0.001. N.D.; not detected.