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Table 2 Effect of Kurozu concentrated liquid (KCL) on 3T3-L1 preadipocyte differentiation and the expression of fatty acid binding protein 2 (aP2) and peroxisome proliferator-activated γ (PPARγ) mRNA during 3T3-L1 preadipocyte differentiation

From: Effects of Kurozu concentrated liquid on adipocyte size in rats

 

Phase I (mean ± SE)

Phase I & II (mean ± SE)

 

Acetic acid

KCL

Acetic acid

KCL

Absorbance

0.139 ± 0.002a

0.124 ± 0.004

0.135 ± 0.001b

0.125 ± 0.001

aP2/β-actin (%)

73.7 ± 0.7b

56.0 ± 2.8

108 ± 1c

32.7 ± 0.5

PPARγ/β-actin (%)

1.74 ± 0.03c

0.943 ± 0.032

1.36 ± 0.04

N.D.

  1. Differentiated cells were stained with Oil Red O and their absorbance was determined at 492 nm. In the determination of mRNA levels, β-actin was used as a loading control. The means and standard errors (SE) are for triplicate experiments. Different superscript letters indicate significant differences at aP < 0.05, bP < 0.01, cP < 0.001. N.D.; not detected.