Flow cytometry Cell cycle analysis, using propidium iodide as a DNA intercalating agent. A) Histogram representative of Control group after 24 h of seed; B) G0/G1 cell cycle arrest induced by 0.8 μM of simvastatin. C) Percentage of G0/G1 cells in witch treatment. D) Increased cell death due to simvastatin effects evaluated by hypodiploid cells amount. Each bar represents the mean, and vertical lines are the standard error of the mean of a representative assay performed in triplicates.