Figure 6
From: Regulations of the key mediators in inflammation and atherosclerosis by Aspirin in human macrophages
Increases of ABCA1 and SR-BI transcripts by ASA in THP-1 derived macrophages. THP-1 cells were cultured in media containing 10% FBS with different concentrations of ASA for 24 h. Total RNAs were isolated and mRNA levels for ABCA (A), SR-BI (B) with Actin as internal controls were examined by RT-PCR analysis. Amplified cDNA products were applied to 1.5% agarose gels, stained with ethidium bromide and visualized under a UV light (A, B). The signal intensities from gels were qualified by the Quantity One software and transformed to the bar graphs (C, D). Results are expressed as mean ±S.D, of three experiments with duplicated samples. * P < 0.05,compared to control(Figure 6C,D).