Figure 3
From: Decreased NPC1L1 expression in the liver from Chinese female gallstone patients
Quantitative immunohistochemistry to detect NPC1L1 from a specimen from a female gallstone-free control using ImageJ. After stripping the original digital image of non-white background (A), colour deconvolution is used to separate the haematoxylin component (B) from the 3',3'-diaminobenzidine (DAB) component (C). The minimum and maximum mean optical density values of eight positively stained cells selected from the DAB analysis are used to set the threshold manually (D) for sample optical density measurement. original magnification 40×.