Effects of FAs on adipocyte morphology and lipid accumulation. The 3T3-L1 pre-adipocytes were incubated in growth medium supplemented with adipogenic factors (520 μM IBMX, 200 nM Dex and 2 μg/mL insulin). FAs (100 μM) of EPA, STA or OLA were included throughout 7 days of adipocyte differentiation. A) Cell morphology of differentiated adipocytes analysed with phase contrast microscopy before (a - d) and after (e - h) Oil Red O stain. B) Stained lipid fraction was measured spectrophotometrically on Day 8 at A520 nm. Values were calculated as percent lipid content versus vehicle control cells treated with 2% BSA + 100% ethanol alone (CTRL) and are expressed as means ± SEM (n = 6). Values with different superscript letters are significantly different at P < 0.05 by Tukey's post hoc test.