Figure 2

Effects of FAs on mRNA expression of (A) adipogenesis; PPARγ and GLUT4, and (B) LD size; Cidea and Perilipin A. Cells were differentiated in the presence of 100 μM FA or vehicle control. EPA, in contrast to STA and OLA, resulted in a significant inhibition of Cidea and PPARγ gene expressions but not Perilipin A or GLUT4. All values are means ± SEM of percent gene expression relative to CTRL, where cDNA levels were normalised by cyclophilin levels (n = 4). Values with different superscript letters are significantly different at P < 0.05 by Tukey's post hoc test.